Elisa Reader And Washer Principle

The elisa reader reads the plate.

Elisa reader and washer principle.

Are available as fixed as well as adjustable volume as well as single channel and multi channel. A microplate washer is used to wash away non specific material in the wells and an absorbance elisa microplate reader detects the color change produced when target antigen is present. The principle in elisa various antigen antibody combinations are used always including an enzyme labeled antigen or antibody and enzyme activity is measured colorimetrically. Along with the enzyme labelling of antigens or antibodies the technique involves following three principles in combination which make it one of the most specific and sensitive than other immunoassays to detect the biological molecule.

And a plate reader software is used to plot standard curves and calculate results. What an elisa reader does. In short it is an antibody test or a test for immune response to things attacking the body such as virus bacteria and allergens. Having the reactants of the elisa immobilized to the microplate surface enables easy separation of bound from non bound material during the assay.

The illustration below shows a workflow for a typical sandwich elisa assay. The test is done in an elisa plate also known as a 96 well plate or microplate. Elisa is a plate based assay technique. Readers need to have appropriate filter 650 nm and 450 nm.

It can be manual system that washes one row or column at a time or semi automated systems that wash one strip or plate at a time or fully automated systems that can process multiple plates. An immune reaction i e.